This stain is used for preparing
specimen for examination of Mycobacterium tuberculosis. The principle has three
solutions which are;
1 Carbol Fuchsine
2 Acid Alcohols
3 Counter Stains
SOLUTUON PREPARATION
SOLUTION ONE
Basic Fuchsin 10g
Phenol Alcohol 45g
Ethyl Alcohol 100ml
Distilled 900ml
PREPARATION PROCEDURE
Dissolved the phenol in the
distill water, leave it, and dissolved basic fuchsin in ethyl alcohol
achievement of this could be done with application of gentile heat. After that,
combine the two solution together, mixed it and left it at room temperature
over night, after that filter, record date label and store. Or weigh the basic
fuchsin and phenol crystal into two liter flask and dissolved with heating on a
boiling water bath. The solution should be should be shaken frequently till
homogenous solution is form. Add alcohol mixed and make it to total volume with
distilled water, left it at room temperature over night filter paper record
date, label and store.
SOLUTION TWO ACID ALCOHOL
1 Hydrochloric Acid 30ml
2 Ethyl Alcohol 97ml
METHOD OF PREPARATION
Mixed hydrochloric acid with
ethyl alcohol record date, label, and store.
SOLUTION THREE (COUNTER STAIN)
Methlene blue or Malachite green
STAINING PROCEDURE
1 Prepare a smear allowed to dry
and fixed by gentile heat.
2 Apply solution one and heat,
keep the staining hot for five minute, but should not allowed to boil and dry.
3 Wash with water.
4 Apply solution two until the
preparation remain colourless.
5 Wash with water.
6 Apply solution three for 20-30
seconds.
7 Wash with water, blot carefully and dry by
gentle heat.
8 Apply oil immersion, and examine with microscope
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