This method and the colorimetric
technique is the same, it just that there is a little slice different.
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| IMMUNOLOGY |
The
sample of the medium together with the indicator in marched against the slide
different is that
| IMMUNOLOGY |
1.
In the first two of the last tube in the comparator
rack, is where 5ml of the media is added to, instead of the three which was explained
in a precious techniques.
2.
Standard volume of indicator is added to the second
tube
3.
At this point the comparator is close and indicator
disc is turn on for pH reading.
4.
Add alkali or acid to the first tube till the colors
are marched.
5.
Calculate the
amount of acid or base added to the medium in the way the colorimetric method
is calculated above all.
| IMMUNOLOGY |
Note: if agar is used the tube
that is makes up of agar and indicator with the one contains only agar must be
left to cool and solidifies before any other things. Reading must not take
place until the media cooled. Before tube is used, they must be washed very
well with distilled water. And finally never compare their color in a direct
sun light, special view can be used for artificial light.

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