MATERIAL
1. microscope
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| MICROBIOLOGY ORINGIN AND TEST |
2. glass slide 22mm
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| MICROBIOLOGY ORINGIN AND TEST |
3. plastacine 2cm long and 1.2cm wide
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| MICROBIOLOGY ORINGIN AND TEST |
4. coverslip
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| LABORATORY EQIUPMENT YOU MUST NO |
SAMPLE
Sample for examination of micro
organism is suspended in a fluid and examine microscopically for identification
of motile bacteria. On this ocation, it could be examine that many bacteria are
seen moving. They move from one position to another. A motile organism is the
organism that change it position do to the present of other organisms. Bellow
are the step taken for the preparation of film for examination of motility of
bacteria.
PROCEDURE
1
On a clean glass slide with a 22mm squire cover slip.
2
Make plastacine ring about 2cm long 1.2cm breath
diameter at the center of the slide, but for alternative well slides. With
depression in the center can be used.
3
Transfer a loopful of the culture colony to the center
of the cover slips.
4
Carefully press the ring of the plastacine onto the
coverslip makes sure the colony in the center of the plastacine so that it dose
not come in contact with the pasticine. It very necessary for the cover slip to
seal with the slide, otherwise false motility is bound to take place.
5
For quick movement of bacteria, invert the slide so
that the cover slip is upper most.
6
Examine by microscopy focusing first onto the edge of
the drop with X40 objective and if focus swinging around use X10 objective to
investigate motility.
7
Discard all the preparation into a jar containing a
disinfectant, care should be taken so that the disinfectant should not
penetrate into the ring and kill the colony.
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