ZIEHL-NEELSEN: This stain is used
for preparation of specimen for examination of mycobacterium tuberculosis; this
method used three solutions, which are;
Carbol Fuchsin
Acid alcohol
Counter stain, which could be
safranin i.e. depend on the laboratory scientist choice.
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| ZIEHL NEELSEN PROCEDURE |
1 Carbol Fuchsin Solution
Basic fuchsin 10g
Phenol crystal 45g
Ethyl alcohol 100ml
Distilled water 900ml
PREPARATION
Dissolved the phenol in the
distilled water, leave it and dissolved basic fuchsin in the ethyl alcohol,
this can be achieved by gentle heat. After that combine the two solutions and
mixed leave it at room temperature overnight.
After that filter and label.
Or weight the basic fuchsin and
phenol crystal into 2 liter flask and dissolved by heating on a boiling water
bath. This process content should be shaken frequently until the solution is
dissolved. Add alcohol mixed and add distilled water, allowed to remain at room
temperature overnight filter with wet filter paper label.
2 Acid alcohols
Hydrochloric Acid 30ml
Ethyl alcohol 97ml
PREPARATION
Mixed hydrochloric acid with
ethyl alcohol record label and store
3 Counter Stain
Methyline blue or malachite green
can be used
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