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August 27, 2016

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COMPLETE BY STEP FOR SPERM ANALYSIS

NORMAL SEMEN CHARACTERISTIC Freshly ejaculated semen should have the following characteristic; 1) Highly viscid opaque and creamy 2) White in colour and have a distinct musky or sickly smell 3) The specimen should liquefy within 20 minute after collection 4) On liquefaction the specimen may change slightly viscous 5) The specimen may be turbid and alkaline with Ph 7.7 6) The total volume is measure, which after 2-5 days abstinence from sex, the volume should be from 2-3ml PARAMETER PROCEDURE 1 TIME OF ARIVAL 2 APEARANCE 3 VISCOSITY/LIQUEFACTION PROCEDURE ...

WIRE LOOP AND HOW TO PRODUCE WIRE LOOP IT IN MICROBIOLOGY LABORATORY

NICHROME WIRE This is straight wire usually circle of approximately 1.5mm and 3mm made up of platinum or nichrome wire with handle which is use for picking colony from culture. Nichrome wire is generally adopted for making wire loop because it is cheaper, elastic and cool faster compared to platinum wire. The wire loop must be renewing time to time. Now there are commercially disposable plastic wire loop available in the market and they are widely used in medical lab now are days. Wire can also be made in the laboratory by the lab scientists, bellow are the procedure for making wire loop in the laboratory; WIRE LOOP Cut wire approximately 10cm, and must be platinum or nichrome wire. Wind the wire once round a metal rod of appropriate diameter. With a pair scissors cut one aim at the wire junction Bend back the wire loop to centre it WIRE LOOP HANDLE Insert into a metal wire holder. STERILIZATION OF WIRE LOOP Hold the loop in the Bunsen flame in ...

PROCEDURE PREPARE THICK FILM FROM CULTURE COLONIES

Sterilized wire loop by passing through flame, allowed it to red hot bring it out and allowed it to cool before picking a colony Take a loop full of culture; transfer to a clean grease free glass slide spread the sample with the loop to form a thick film of a fluid. sterilized the wire loop Leave the film to air dry without heating and pass through the flame 3 time to kill the bacteria and fixed it to a slide. the slide should be allowed to cool CULTURE COLONIE stain with the right stain examine with microscopy, by microscopic procedure

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