HOW TO ISOLATE A SINGLE COLONY

For isolation of a single colony, there are step required to inoculate the petri dish before the mission can be accomplished. The steps are;

 

1. Sterile wire loop, take a loopful specimen and inoculate over area A.
2. Sterilize the wire loop allowed it to cool, pick a loopful specimen and inoculate it over area B
3. Sterilize it and allowed to cool, and pick a again inoculate it over area D

Continuously if there is space, after the inoculation incubate the plate at a required temperature. Make sure that no area of inoculation is left unused, and extreme care must be taken in other no to cross the previously inoculated area.

Another method can be used for substitution of the one explain above, on this method 3mm glass rod spreader is used. The glasses are sterilized either by boiling or by raping it in a klaft paper and place in an oven at temperature at 160 ºC for one hour. After this small aliquot is place on the media and smear over the surface of the media by a spreader, after another plate is prepared by using the same first inoculation spreader and inoculated on the plate.

Note:  any specimen that remains in the first plate must be taken to second place in these there an expectation of the same colonies to growth in both inoculated plate.

In other to obtain a specific result it is necessary that the surface of the media on the plate must be dry. Plate should be dry by putting the lid surface in an incubator set at a temperature of 37 ºC, these should be set by making the media in the dish angle downward. These could be within the plate or on the edge of the lid. In another way media plate are dried through the previous guideline and inserted in the front of a thin plate flow area in an incubator to achieve the desired result.