GENERAL METHOD OF SMEAR MAKING IN THE MEDICAL LABORATORY

1. Use a clean grease free glass slide
2. Mark the slide with a written material such as diamond grease or pencil, but pencil is easily rube away.
3. Use only one smear per slide and keep the slides well transfer of acid fast organism onto another slide. Throw the slide away after used
4. Make fairly heavy smear from liquid cultures and thin smear from solid media culture.
5. Aseptic precaution must be observed during manipulation of the culture tube or plate.
6. Do not use water taken from rubber attached to the taps for making smear as organism may be transfer from, the rubber.
7. Disinfect spill immediately.

LIQUID MEDIA SPECIMEN PREPARATION STEP

1. Sterilized wire loop by passing through flame, allowed it to red hot bring it out and allowed it to cool before picking a colony
2. Take a loop full of culture; transfer to a clean grease free glass slide spread the sample with the loop to form a thick film of a fluid.
3. sterilized the wire loop
4. Leave the film to air dry without heating and pass through the flame 3 time to kill the bacteria and fixed it to a slide.
5. the slide should be allowed to cool
6. stain with the right stain
7. examine with microscopy, by microscopic procedure.

SOLID MEDIA SPECIMEN PREPARATION STEP

1.        sterilized the wire loop

2.        place a drop of distilled water on a grease free clean slide

3.        sterilized the loop

4.        with wire loop transfer a loopful colony to be examine on a slide

5.        mix it with the water to give you a uniformed films, sterilized the wire loop

6.        Allowed the film to air dry and fix with the appropriate stain and take it to microscope for examination via microscopic procedure.

MOTILE BACTERIA

Sample for examination of micro organism is suspended in a fluid and examine microscopically for identification of motile bacteria. On this ocation, it could be examine that many bacteria are seen moving. They move from one position to another. A motile organism is the organism that change it position do to the present of other organisms. Bellow are the step taken for the preparation of film for examination of motility of bacteria.

1              On a clean glass slide with a 22mm squire cover slip.

2              Make plastacine ring about 2cm long 1.2cm breath diameter at the center of the slide, but for alternative well slides. With depression in the center can be used.

3              Transfer a loopful of the culture colony to the center of the cover slips.

4              Carefully press the ring of the plastticine onto the coverslip makes sure the colony in the center of the pasticine so that it dose not come in contact with the pasticine. It very necessary for the cover slip to seal with the slide, otherwise false motility is bound to take place.

5              For quick movement of bacteria, invert the slide so that the cover slip is upper most.

6              Examine by microscopy focusing first onto the edge of the drop with X40 objective and if focus swinging around use X10 objective to investigate motility.

7              Discard all the preparation into a jar containing a disinfectant, care should be taken so that the disinfectant should not penetrate into the ring and kill the colony.

WET PREPARATION

1              Mix the specimen in a small drop of saline, iodine or stain on a glass microscopic slide.

2              Place cover slip onto the suspension and care should be taken so that the specimen should not escape through the edge of the cover slip.

3              Paint the cover slip edge with paraffin wax or Vaseline so that it prevents overflow or evaporation of the sample and examine microscopically.