AURAMINE STAINING SEE HOW TURBACURLOSIS LOOK WHEN WITH MICROSCOPE

This method is useful for examination of acid fast bacilli in a sputum specimen, by the use of florescence microscopy. This method also has solution one to three;

TB ON URAMINE

TB ON URAMINE

SOLUTION ONE (PRIMARY STAIN)

Uramine                            0.3g

Phenol crystal                   3.0g

Distilled water                  97ml

PREPARATION

Dissolved phenol in distilled water by gentile heat, add uramine gradually by continues shaken till the solution form homogeneously, filter record date label and store in a dark stoppered battle, and can be left for three weeks.

Note: care should be taken in handling the solution, because phenol is corrosive.

SOLUTION TWO (DECOLORISER)

Sodium chloride                               0.5g

Hydrochloric     acid                        0.5ml

Ethyl alcohol                                    100ml

PREPARATION METHOD

Combine the ethyl alcohol with hydrochloric acid and dissolved sodium chloride in the solution record date label and store.

Note: the solution should be handled carefully, because it is highly corrosive and inflammable.

SOLUTION THREE (COUNTER STAIN)

Potassium permanganate 1%solution.